A Sample Image is an image file you will use as a template to define your zones. It should be representative of the images you intend to process with the zones you have defined. Representative image means that the image is the same DPI and Pixel size as all the documents you intend to process. And the zone locations defined on the sample image should be at the same location for all the documents you intend to process with your defined zones.
To load a new Image File, click Get Sample Image link.

Original - display the image in its actual size
AutoSize - display the image to fit in the available space
ScaleWidth - display the image so that it uses the full width of the available space
Zoom In - enlarge the image
Zoom Out - reduce the image